A. 1000-Hours of Raw Materials Screening

We search for ingredients that possess the efficacy our product aims for, selecting them based on scientific literature and research papers.

B. Securing Safety Data

We review whether the ingredient is suitable for use in cosmetics. Ingredients excluded in this process are mainly those for which safety data is unavailable or those only ideal for pharmaceutical use.

C. 5-Times of Raw Materials Quality Verification

We aim only for high-quality ingredients; therefore, we verify them in various ways.

C1. Non-Destructive Automatic Sampling

This method automatically collects samples without damaging the product, preparing samples for accurate analysis while maintaining product quality.

C2. Purity Testing

GCMS, LCMS, HPLC, UPLC: These technologies are used to measure the purity of compounds, and each analysis technique is designed to be suitable for different types of compounds. GCMS and LCMS use gas and liquid chromatography, respectively, while HPLC and UPLC analyze liquid samples under high pressure to separate and quantify components.

C3. Structural Analysis

NMR, Dispersive X-ray Diffraction Analysis: NMR is used to elucidate the molecular structure of compounds, while dispersive X-ray diffraction analysis is used to analyze the microstructure of materials, including crystal structures. These methods are essential for accurately understanding the molecular and crystal structures of cosmetic raw materials.

C4. Mass Analysis

MALDI TOF Qualitative, QTRAP Quantitative: MALDI TOF is used for rapid identification of specific molecules in large samples for qualitative analysis, while QTRAP is used for quantitative analysis of specific compounds. These technologies measure the exact mass of cosmetic raw materials to identify components and determine quantities.

C5. Protein Interaction Analysis

MST and ITC: MST (Microscale Thermophoresis) and ITC (Isothermal Titration Calorimetry) are used to analyze the interaction between proteins and ligands to understand intracellular signaling processes. These methods are important for understanding how cosmetic raw materials act within cells.

D. 100-Times of Verification of Ingredient Efficacy

D1. In Silico Experiments

To minimize animal and cell experiments in the early stages of research, molecular dynamics, toxicity prediction, and skin permeability prediction experiments are conducted through computer simulations for molecular prediction modeling.

D2. In Vitro Experiments

Once basic research data on selected ingredients is compiled, laboratory tests at the cellular level are conducted to verify the efficacy of the ingredients. Through these experiments, the impact of the ingredients on skin cells can be precisely evaluated.

E. 500-Times of Formulating

Based on the laboratory test results, we create a draft of the product formula by blending the ingredients at the optimal combination and concentration. This formulation process takes into account not only the efficacy of the product but also consumer experience factors such as usability and texture.

F. Safety and Stability Tests

Once the product formula is determined, safety and stability tests are conducted to evaluate the long-term effects of product use and product changes under storage conditions. This is to ensure that the product does not cause adverse effects on the skin and that the quality of the product is maintained.